Enzymatic digestion and chromatographic analysis of arsenic species released from proteins
نویسندگان
چکیده
منابع مشابه
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF SULFUR MUSTARD REACTION WITH AMINO ACIDS AND PROTEINS
The interaction of sulfur mustard with aminoacids and proteins has been investigated in this study. Rats were injected with sublethal doses of sulfur mustard subcutaneously and intraperitoneally. At different time intervals, plasma and urine samples were collected. The binding affinity of sulfur mustard with urinary and plasma proteins and enzymes was studied for the first time using non-i...
متن کاملReovirus: analysis of proteins from released and cell-associated virus.
The proteins of purified preparations of reovirus type 3 have been examined using polyacrylamide gel electrophoresis. Preparations of cell-associated virus grown with foetal calf serum show three major peaks, 3., /z, o-, and several minor peaks. Preparations of released virus grown without foetal calf serum show only the three major peaks, suggesting that the minor peaks are not virus proteins....
متن کاملHigh-performance liquid chromatographic measurement of amiodarone and desethylamiodarone in small tissue samples after enzymatic digestion.
A method is described for the measurement of amiodarone and desethylamiodarone in small tissue samples. With the exception of fat, for which lipase is used, the tissues are digested with a proteolytic enzyme. After the addition of an internal standard the analytes are extracted from the homogeneous digest into an organic solvent and measured by high-performance liquid chromatography (HPLC) with...
متن کاملGas-liquid chromatographic measurement of lignocaine in small samples of canine myocardium after enzymatic digestion.
A method is described whereby lignocaine can be measured in small samples of canine myocardium. A proteolytic enzyme is used to digest the tissue, and the drug, together with an internal standard, is subsequently extracted into an organic solvent and measured by gas-liquid chromatography. The method is reproducible using tissue samples as small as 50 mg and appears to be suitable for the measur...
متن کاملChromatographic Analysis of the Flavonoids from Robinia Pseudoacacia Species
THE PURPOSE OF THIS STUDY WAS THE QUALITATIVE AND QUANTITATIVE ANALYSIS OF FLAVONOIDS FROM ROBINIA PSEUDOACACIA USING TWO DIFFERENT TECHNIQUES OF ANALYSIS: Thin Layer Chromatography (TLC) and TLC coupled with photo-densitometry. The results obtained by chromatographic analysis showed a higher concentration of flavonoids in flowers than in leaves. The flowers harvested in the plains have a highe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2009
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2009.03.015